Lactate Dehydrogenase B/LDHB Antibody

• Catalog Number: orb381078
• Category: Antibodies
• Description: Lactate Dehydrogenase B/LDHB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 36638 MW
• UniProt ID: P07195
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: L-lactate dehydrogenase B chain;LDH-B;1.1.1.27;LDH
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-LDHB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB:Lane 1:human HeLa cell; 2:human placenta tissue; 3:human PC-3 cell; 4:monkey COS-7 cell; 5:human HEK293 cell; 6:human A549 cell; 7:monkey kidney tissue; 8:rat kidney tissue; 9:rat heart tissue; 10:mouse kidney tissue; 11:mouse heart tissue.

IF analysis of LDHB using anti-LDHB antibody.LDHB was detected in immunocytochemical section of A431 cell.

IHC analysis of LDHB using anti-LDHB antibody. LDHB was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of LDHB using anti-LDHB antibody. LDHB was detected in a paraffin-embedded section of rat spleen tissue.

IHC analysis of LDHB using anti-LDHB antibody. LDHB was detected in a paraffin-embedded section of human tonsil tissue.