La Crosse virus, G1 envelope glycoprotein antibody

• Catalog Number: orb1152483
• Category: Antibodies
• Description: Mouse monoclonal antibody to La Crosse virus, G1 envelope glycoprotein
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 807.31
• Tested applications: ELISA, FC, IP, WB
• Reactivity: Virus
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising BALB/c mice with La Crosse viruses and the harvested spleens were fused with mouse P3X63Ag8.653 myeloma cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: La Crosse virus, G1 envelope glycoprotein
• UniProt ID: Q8JPR1
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: LCV G1; G1 envelope glycoprotein; G1 glycoprotein;
• Note: For research use only
• Application notes: In the original publication, this antibody, together with other 22 clones against the the G1 and N proteins of LaCrosse and Tahyna, was characterised by the ELISA assays, immunoprecipitation, neutralisation tests, and hemagglutination inhibition tests (Gonzalez-Scarano et al, 1982). It was used for antigenic taxonomy of Califmia serogroup viruses and for the identification of the California serogroup viruses of North America (Gonzalez-Scarano et al, 1982). It was also used, together with the other anti-La Crosse mAb clones 807.35 and 807.27, in the neutralisation assays of murine leukemia virus pseudotypes of La Crosse and Hantaan Bunyaviruses to validate a system for analysis of cell tropism (Ma et al, 1999). Furthermore, a single immunisation with this antibody was reported to result in a robust immune response and protection against La Crosse virus (Pekosz et al, 1995). Recently, this antibody has been reported in various FACS analyses, for example, to demonstrate that mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066–1087) as the fusion peptide (Plassmeyer et al, 2007), and to suggest that the fusion peptide of La Crosse virus Gc is a determinant of properties associated with neurotoxicity (Soldan et al, 2010).
• Expiration Date: 12 months from date of receipt.