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    L1CAM antibody

    Catalog Number: orb315797

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb315797
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to L1CAM
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat, Zebrafish
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human L1CAM. The exact sequence is proprietary.
    Dilution rangeWB: 1-500:1000, IF/ICC: 1-100:500
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetL1CAM
    Entrez3897, 50687
    UniProt IDQ05695, P32004, P11627
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti CAML1 antibody, anti MIC5 antibody, anti Neur
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    L1CAM antibody

    Western blot analysis of L1CAM expression in HEK293T (A), A549 (B) whole cell lysates. (Predicted band size: 140 kD; Observed band size: 140 kD)

    L1CAM antibody

    Immunohistochemical analysis of L1CAM staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    L1CAM antibody

    Immunofluorescent analysis of L1CAM staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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