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L1CAM Antibody / NCAM-L1 / CD171
Catalog Number: orb2636045
| Catalog Number | orb2636045 |
|---|---|
| Category | Antibodies |
| Description | L1 (L1 cell adhesion molecule) also known as CAML1, CD171, and HSAS is an adhesion molecule which plays an important role in the nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). This mouse monoclonal antibody recognizes the sequence immediately NH2-terminal to the tyrosine-based motif and binds L1 only when Y1176 is dephosphorylated. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain. |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | A recombinant fragment (within amino acids 600-900) of human L1CAM protein was used as the immunogen for the recombinant CD171 antibody. |
| UniProt ID | P32004 |
| Tested applications | IHC-P |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application notes | Optimal dilution of the recombinant CD171 antibody should be determined by the researcher. |
| Antibody Type | Primary Antibody |
| Clone Number | L1CAM/9267R |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Hazard Information | This recombinant CD171 antibody is available for research use only. |
| Note | For research use only |
IHC staining of FFPE human brain tissue with recombinant CD171 antibody (clone L1CAM/9267R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD171 antibody (clone L1CAM/9267R) as confirmation of integrity and purity.
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