KRT76 Antibody

• Catalog Number: orb1252385
• Category: Antibodies
• Description: KRT76 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRTH/1076
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT76
• UniProt ID: Q01546
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: KRT2B, KRT2P, Keratin, type II cytoskeletal 2 oral
• Note: For research use only
• Application notes: ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin paraffin human skin stained with Basic Cytokeratin antibody (KRTH/1076).

Formalin paraffin rat stomach stained with Basic Cytokeratin antibody (KRTH/1076).

Formalin paraffin rat oviduct with Basic Cytokeratin antibody (KRTH/1076).

SDS-PAGE Analysis of Purified, BSA-Free Basic Cytokeratin Antibody (clone KRTH/1076). Confirmation of Integrity and Purity of the Antibody.