KRT7 Antibody Cocktail / Cytokeratin 7

• Catalog Number: orb749627
• Category: Antibodies
• Description: It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant full-length human KRT7 protein (KRT7/760) and OTN 11 cells (OV-TL12/30) were used as the immunogen for the Cytokeratin 7 antibody cocktail.
• UniProt ID: P08729
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Cytokeratin 7 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: KRT7/760 + OV-TL12/30
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the Cytokeratin 7 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This Cytokeratin 7 antibody cocktail is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody cocktail (green, clones KRT7/760 + OV-TL12/30) and Reddot nuclear counterstain (red).

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 7 antibody (KRT7/760 + OV-TL12/30)

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 antibody (KRT7/760 + OV-TL12/30)

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody cocktail (clones KRT7/760 + OV-TL12/30).