KRT19 Antibody / Cytokeratin 19

• Catalog Number: orb2645259
• Category: Antibodies
• Description: Cytokeratin-19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM561
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human mammary epithelial organoids were used as the immunogen for this Cytokeratin 19 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cytokeratin 19 antibody is available for research use only.
• UniProt ID: P08727
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561, green) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (clone SPM561).

Western blot testing of human MCF7 cells with Cytokeratin 19 antibody (clone SPM561). Predicted molecular weight ~43 kDa.

Flow cytometry testing of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561); Red=isotype control, Blue= Cytokeratin 19 antibody.