KRT13 antibody

• Catalog Number: orb20058
• Category: Antibodies
• Description: Goat polyclonal antibody to KRT13
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 49.6; 45.8
• Target: KRT13
• Entrez: 3860
• Protein Sequence: TSNASGRRTSDVRRP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KRT13 antibody, anti keratin 13 antibody, ant
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Tonsil shows strong layered, filamentous staining. Recommended concentration, 2-4ug/ml.WB: Approx. 55kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149(Pt 4):961-71.; PMID: 12686638). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20058 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20058 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.