KRT10 Antibody

• Catalog Number: orb1252499
• Category: Antibodies
• Description: KRT10 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT10/844
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG1, kappa
• Immunogen: Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Keratin 10 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT10
• UniProt ID: P13645
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Keratin, type I cytoskeletal 10, Cytokeratin-10, C
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Keratin 10 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human skin with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

IHC testing of FFPE human skin with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

IHC testing of FFPE human bladder carcinoma with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Keratin 10 Antibody (clone KRT10/844). Confirmation of Integrity and Purity of the Antibody.