KRT10 Antibody Cocktail / Cytokeratin 10

• Catalog Number: orb385997
• Category: Antibodies
• Description: This mAb cocktail recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT10/844 + KRT10/1275
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody cocktail.
• Dilution range: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Keratin 10 antibody cocktail is available for research use only.
• UniProt ID: P13645
• Storage: Store the Keratin 10 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Keratin 10 antibody cocktail should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human skin using Cytokeratin 10 Cocktail antibody