KNTC1 antibody

• Catalog Number: orb101601
• Category: Antibodies
• Description: KNTC1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Human, Rat, Sheep
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human KNTC1/Kinetochore (1881-1980/2209 aa)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 251 kDa
• Target: KNTC1
• UniProt ID: P50748
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: HsROD; Kinetochore associated 1; KNTC1; ROD; Rough
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Huang, Houfeng et al. Knockdown of KNTC1 Inhibits the Proliferation, Migration and Tumorigenesis of Human Bladder Cancer Cells and Induces Apoptosis Crit Rev Eukaryot Gene Expr, 31, 49-60 (2021)

PubMed: 33639055

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; An