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Catalog Number | orb548364 |
---|---|
Category | Antibodies |
Description | KMT5A / SETD8 / Pr-SET7 Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | ADHO-11 |
Tested applications | FC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human KMT5A / SETD8 / Pr-SET7 Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Dilution range | WB 1:1000-1:5000FC 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | Q9NQR1 |
Sensitivity | < 50pg/ml |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of KMT5A expression in (1) 293T cell lysate; (2) NIH/3T3 cell lysate using anti-KMT5A antibody (orb548364). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT5A antigen affinity purified polyclonal antibody (Catalog # orb548364) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for KMT5A.
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