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Catalog Number | orb1263774 |
---|---|
Category | Antibodies |
Description | KLF6 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit Ig |
Immunogen | This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 32 kDa |
Target | KLF6 |
UniProt ID | Q99612 |
NCBI | Q99612 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Krueppel-like factor 6, B-cell-derived protein 1, Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of KLF6 Antibody in mouse stomach tissue lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of widr cells using KLF6 Antibody (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of Hela cell stained with KLF6 Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.
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