KLF4 antibody

• Catalog Number: orb18840
• Category: Antibodies
• Description: Goat polyclonal antibody to KLF4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 50.1
• Target: KLF4 / GKLF
• Entrez: 9314
• Protein Sequence: AVSDALLPSFST
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KLF4 antibody, anti GKLF antibody, anti Krupp
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Experiments gave strong bands at 75-90kDa and a faint 50-kDa band in Human Duodenal lysate after 0.1 μg/ml antibody staining. These bands correspond to earlier findings in literature (Piccinni et al, J. Biol. Chem., Vol. 279 (10), 9103-9114). All these bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18840 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.