KLF4 antibody

• Catalog Number: orb1474158
• Category: Antibodies
• Description: Mouse monoclonal antibody to KLF4
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human KLF4. The exact sequence is proprietary.
• Dilution range: WB (1/1000 - 1/2000)
• Form/Appearance: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: KLF4
• Entrez: 9314
• UniProt ID: O43474
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: EZF; GKLF; Krueppel-like factor 4; Epithelial zinc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of KLF4 expression in recombincant protein (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 62 kD)

Immunofluorescent analysis of KLF4 staining in Hela cells transfected with pMX constructs. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Hela cells using Anti-KLF4 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF594 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.