KITLG / SCF Antibody

• Catalog Number: orb1539117
• Category: Antibodies
• Description: KITLG / SCF Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Immunogen: E.coli derived recombinant Human SCF.
• Dilution range: ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Conjugation: Unconjugated
• Target: KITLG / SCF
• Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2
• Alternative names: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7
• Note: For research use only
• Application notes: Resuspension: Reconstitute with sterile water. Further information: Immunohistochemistry: LS-B8209 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hSCF (100 ng/ml), a concentration of 008-012 ug/ml of this antibody is required. ELISA: To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (LS-C104803) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.