Kitlg Antibody

• Catalog Number: orb1272656
• Category: Antibodies
• Description: Kitlg Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Rat
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat IP-10. Rat IP-10 specific antibody was purified by affinity chromatography employing an immobilized Rat IP-10 matrix.
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA:Indirect:To detect rat SCF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. SandwichTo detect rat SCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat SCF (38-258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. Western Blot:To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: Kitlg
• UniProt ID: P21581
• NCBI: P21581
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Mgf, SCF, Kitl, Mgf, Kit ligand, Hematopoietic gro
• Note: For research use only
• Application notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA:Indirect:To detect rat SCF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. SandwichTo detect rat SCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat SCF (38-258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. Western Blot:To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect rat SCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat SCF (orb1272649) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.

To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.