KIF2C Antibody / MCAK

• Catalog Number: orb1824567
• Category: Antibodies
• Description: Kinesin family member 2c (KIF2C), alternately known as mitotic centromere associated kinesin (MCAK) and Kinesin like protein 6 (KSL6), is a member of the kinesin-like family of proteins. KIF2C is a cytoplasmic and nuclear protein, present throughout the cell cycle. KIF2C associates with the centromere early in prophase, and disassociates after telophase. KIF2C is abundant in thymus and testis, and present at lower levels in small intestine, the mucosal lining of the colon, and placenta. Human KIF2C maps to chromosome 1p34.1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KIF2C/4704
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 500-700) from the human protein was used as the immunogen for the Kinesin like protein 6 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Kinesin like protein 6 antibody is available for research use only.
• UniProt ID: Q99661
• Storage: Aliquot the Kinesin like protein 6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human testis tissue lysate with Kinesin like protein 6 antibody (clone KIF2C/4704) at 2 ug/ml. Predicted molecular weight 76-81 kDa.

IHC staining of FFPE human testis tissue with Kinesin like protein 6 antibody (clone KIF2C/4704) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testis tissue with Kinesin like protein 6 antibody (clone KIF2C/4704) at 2 ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Kinesin like protein 6 antibody (clone KIF2C/4704) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using Kinesin like protein 6 antibody (clone KIF2C/4704). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.