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Catalog Number | orb546361 |
---|---|
Category | Antibodies |
Description | KIF15 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human KIF15 recombinant protein (Position: M1201-S1388). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 150-160 kDa |
UniProt ID | Q9NS87 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Kinesin-like protein KIF15; Kinesin-like protein 2 Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of SiHa cells using anti-KIF15 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U20S cells using anti-KIF15 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of KIF15 using anti-KIF15 antibody.Lane 1:human HL-60 cell; 2:human U20S cell; 3:human K562 cell; 4:human PC-3 cell; 5:human HepG2 cell; 6:human HeLa cell.
IF analysis of KIF15 using anti-KIF15 antibody. KIF15 was detected in immunocytochemical section of A431 cells.
IHC analysis of KIF15 using anti-KIF15 antibody. KIF15 was detected in paraffin-embedded section of human colon cancer tissue.
IHC analysis of KIF15 using anti-KIF15 antibody. KIF15 was detected in paraffin-embedded section of rat testis tissue.
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