Ki-67 Mouse Monoclonal Antibody

• Catalog Number: orb500706
• Category: Antibodies
• Description: Ki-67 Mouse Monoclonal Antibody
• Target: MKI67
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human Ki-67
• UniProt ID: P46013
• MW: 358 kDa
• Tested applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Clone Number: 6B9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Antigen identified by monoclonal antibody Ki 67; A
• Research Area: Cancer Research, Cell Cycle, Neurogenesis, Neurosc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hela. Primary Antibody (green line): Mouse Anti-Ki-67 antibody (orb500706), dilution: 1 ug/Test, Secondary Antibody: Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.