Cytokeratin 18/KRT18 Mouse Monoclonal Antibody

SKU: orb3085300

Description

The Cytokeratin 18 KRT18 Mouse Monoclonal Antibody (Carrier-free) is a Carrier-free antibody raised in mouse. It is immunogen affinity purified and suitable for use in FC, ICC, IF, IHC, IHC-Fr, and WB applications. This antibody targets the Keratin, type I cytoskeletal 18 in Human samples.

Research Area

Cancer Biology, Epigenetics & Chromatin, Molecular Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: B9K8
• Immunogen: E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.
• Target: Keratin, type I cytoskeletal 18
• Molecular Weight: 48 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK 18; CK18; CYK18; Cytokeratin 18; K18; keratin 18; KRT18

Quick Database Links

Gene Symbol

Keratin, type I cytoskeletal 18

UniProt

P05783