Keratin 6 Antibody / Cytokeratin 6

• Catalog Number: orb2640061
• Category: Antibodies
• Description: This mAb recognizes a protein of 56kDa, identified as Cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT6/1702
• Tested applications: IHC-P
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant human CK6 protein was used as the immunogen for the Cytokeratin 6 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cytokeratin 6 antibody is available for research use only.
• UniProt ID: P02538
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Clathrin Light Chain antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human bladder carcinoma with Cytokeratin 6 antibody (clone KRT6/1702). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.