KRT10 Antibody

SKU: orb1252499

Description

KRT10 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.1-0.2 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Keratin 10 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: KRT10/844
• Immunogen: Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.
• Target: KRT10
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP

IHC testing of FFPE human skin with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

IHC testing of FFPE human skin with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

IHC testing of FFPE human bladder carcinoma with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Keratin 10 Antibody (clone KRT10/844). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

KRT10

UniProt

P13645