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Catalog Number | orb1474410 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to KDM5C |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, WB |
Reactivity | Human |
Immunogen | Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E.coli |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) |
Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Conjugation | Unconjugated |
Target | KDM5C |
Entrez | 8242 |
UniProt ID | P41229 |
Source | Mouse |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Alternative names | DXS1272E; JARID1C; SMCX; XE169; Lysine-specific de Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of KDM5C expression in Hela (A), 293T (B), T47D (C), MCF7 (D) whole cell lysates. (Predicted band size: 176 kD; Observed band size: 220 kD)
Immunofluorescent analysis of KDM5C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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