KDM3A Antibody

• Catalog Number: orb1239525
• Category: Antibodies
• Description: KDM3A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Anti-JMJD1A antibody (orb1239525) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50-100 of JMJD1A.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 147kDObserved: 147kD
• Target: KDM3A
• UniProt ID: Q9Y4C1
• NCBI: NP_060903
• Storage: JMJD1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: JMJD1A Antibody: TSGA, JMJD1, JHDM2A, JHMD2A, JMJD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD1A, orb1239525 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD1A, orb1239525 (2 µg/mL), JMJD1A (2 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human HepG2 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD1A, orb1239525 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of JMJD1A in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-JMJD1A antibody (orb1239525) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of JMJD1A in Rat Liver Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling JMJD1A with orb1239525 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).