KDM1 Antibody

• Catalog Number: orb1239604
• Category: Antibodies
• Description: KDM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-LSD1 antibody (orb1239604) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human LSD1. The immunogen is located within amino acids 40-90 of LSD1.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.2 - 2 μg/mL; IHC-P: 2 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 93kDObserved: 110 kD
• Target: KDM1
• UniProt ID: O60341
• NCBI: NP_001009999
• Storage: LSD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604 (0.2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604 (0.2 µg/mL), LSD1 orb1274534 (1 µg/mL), beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604 (0.2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse P815 Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LSD1 orb1239604, (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of LSD1 in Human Breast Carcinoma Tissue. Immunohistochemical analysis of paraffin-embedded human breast carcinoma Tissue using anti-LSD1 antibody (orb1239604) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of LSD1 in human breast carcinoma Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast carcinoma Tissue labeling LSD1 with orb1239604 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green/blue).