KDEL antibody

• Catalog Number: orb151345
• Category: Antibodies
• Description: Rabbit polyclonal to KDEL (HRP). The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER..
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KDEL containing peptide immunogen
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: HRP
• Target: KDEL
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: Lys Asp Glu Leu antibody
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
• Expiration Date: 12 months from date of receipt.