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Catalog Number | orb546350 |
---|---|
Category | Antibodies |
Description | KCC2/SLC12A5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human KCC2/SLC12A5 recombinant protein (Position: A228-N833). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 126 kDa |
UniProt ID | Q9H2X9 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Solute carrier family 12 member 5; Electroneutral Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of SLC12A5 using anti-SLC12A5 antibody.Lane 1:human U2OS cell;2:human HepG2 cell;3:human HEK293 cell;4:human K562 cell;5:human PC-3 cell;6:mouse SP20 cell.
IHC analysis of SLC12A5 using anti-SLC12A5 antibody.SLC12A5 was detected in paraffin-embedded section of human tonsil tissues.
IHC analysis of SLC12A5 using anti-SLC12A5 antibody.SLC12A5 was detected in paraffin-embedded section of rat brain tissues.
IHC analysis of SLC12A5 using anti-SLC12A5 antibody.SLC12A5 was detected in paraffin-embedded section of human Ovarian cancer tissues.
IHC analysis of SLC12A5 using anti-SLC12A5 antibody.SLC12A5 was detected in paraffin-embedded section of human placenta tissues.
IHC analysis of SLC12A5 using anti-SLC12A5 antibody.SLC12A5 was detected in paraffin-embedded section of mouse brain tissues.
IHC analysis of SLC12A5 using anti-SLC12A5 antibody.SLC12A5 was detected in paraffin-embedded section of human sarcoma tissues.
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