Kappa Light Chain Antibody

• Catalog Number: orb248400
• Category: Antibodies
• Description: This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant human Kappa light chain was used as the immunogen for this anti-Kappa light chain antibody.
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-Kappa light chain antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: KLC709
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the anti-Kappa light chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This anti-Kappa light chain antibody is available for research use only.
• Note: For research use only
• Entrez: 3514

IHC testing of human tonsil stained with anti-Kappa light chain antibody (clone KLC709).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using anti-Kappa light chain antibody (clone KLC709). These results demonstrate the foremost specificity of the KLC709 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free anti-Kappa light chain antibody (clone KLC709) as confirmation of integrity and purity.