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Kappa Light Chain Antibody

Catalog Number: orb248398

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb248398
CategoryAntibodies
DescriptionThis antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenRecombinant human Kappa light chain was used as the immunogen for this Kappa antibody.
Tested applicationsFACS, IHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Kappa antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberKLC264
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis Kappa antibody is available for research use only.
NoteFor research use only
Kappa Light Chain Antibody

IHC testing of human tonsil stained with Kappa antibody (clone KLC264). Note cell membrane & cytoplasmic staining.

Kappa Light Chain Antibody

Western blot analysis of human spleen tissue and Kappa antibody (clone KLC264).

Kappa Light Chain Antibody

Flow cytometry testing of PFA-fixed human Raji cells with Kappa antibody (clone KLC264); Red = isotype control, Blue = Kappa antibody.

Kappa Light Chain Antibody

SDS-PAGE analysis of purified, BSA-free Kappa antibody (clone KLC264) as confirmation of integrity and purity.

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