Kappa (L chain) antibody (FITC)

• Catalog Number: orb43639
• Category: Antibodies
• Description: Mouse Monoclonal to Kappa (L chain).
• Clonality: Monoclonal
• Clone Number: A8B5
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: Ig Kappa Light Chain
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Kappa light chains antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human Ig kappa light chain positive B-lymphocytes (red-filled) from Ig kappa light chain negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).