K77 Antibody

• Catalog Number: orb1251983
• Category: Antibodies
• Description: K77 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ACCK1-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgG1, kappa
• Immunogen: An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/ml (3)Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Acidic Cytokeratin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. This antibody will detect the following cytokeratins (CK): CK10: 56kDa; CK14: 50kDa; CK15: 50kDa; CK16: 48kDa; CK19: 40kDa.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: K77
• UniProt ID: Q7Z794
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Western blot: 1-2 ug/ml (3)Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Acidic Cytokeratin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. This antibody will detect the following cytokeratins (CK): CK10: 56kDa; CK14: 50kDa; CK15: 50kDa; CK16: 48kDa; CK19: 40kDa.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil with Acidic Cytokeratin antibody.

IHC testing of FFPE rat stomach with Acidic Cytokeratin antibody.