JNK1/2/3 (Phospho-T183/Y185) antibody

• Catalog Number: orb315601
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JNK1/2/3 (Phospho-T183/Y185)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human JNK1/2/3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAPK8; MAPK9; MAPK10
• Entrez: 116554, 25272, 26419, 26420, 5602, 5599, 50658, 5601
• UniProt ID: Q9WTU6, P49186, P49185, P45984, P49187, P53779, Q91Y86, P45983, Q61831
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti MAPK8 antibody, anti JNK1 antibody, anti PRKM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of JNK1/2/3 (Phospho-T183/Y185) expression in BV2 (A), MCF7 (B), HCT116 (C), U87MG (D) whole cell lysates. (Predicted band size: 48; 52 kD; Observed band size: 46; 54 kD)

Immunofluorescent analysis of JNK1/2/3 (Phospho-T183/Y185) staining in LS8 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).