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Catalog Number | orb1733245 |
---|---|
Category | Antibodies |
Description | JDP2 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human, Mouse |
Immunogen | between 50 and 100 |
Concentration | 400 µg/ml |
Dilution range | WB: 1:1000, IHC: 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections, ICC: 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE cell sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | JDP2 |
UniProt ID | Q8WYK2 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | JUNDM2; progesterone receptor co-activator; jun di Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse JDP2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and RenCa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JDP2 by western blot. Samples: Whole cell lysate (25 µg) from MCF-7, HeLa, HaCaT, and DU145 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunohistochemistry. Sample: FFPE section of mouse RenCa tumor. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human JDP2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunocytochemistry. Sample: FFPE section of human FaDu cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733245). Secondary: HRP-conjugated goat anti-rabbit IgG.
FC, ICC, IHC, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IHC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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