JARID1D/SMCY Antibody

• Catalog Number: orb1527857
• Category: Antibodies
• Description: JARID1D/SMCY Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 1490 and 1540
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: JARID1D/SMCY
• UniProt ID: Q9BY66
• NCBI: NP_004644.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HY; lysine (K)-specific demethylase 5D; Smcy homol
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SMCY/JARID1D by western blot. Samples: Whole cell lysate (15 µg) from Jurkat, HDLM-2, A-549, 786-O, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMCY/JARID1D antibody orb1527857 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SMCY/JARID1D by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMCY/JARID1D antibody orb1527857 used for IP at 3 µg per reaction.