JAM-A Antibody

• Catalog Number: orb1525814
• Category: Antibodies
• Description: JAM-A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 249 and 299
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: JAM-A
• UniProt ID: Q9Y624
• NCBI: NP_058642.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: junctional adhesion molecule 1; platelet F11 recep
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human JAM-A by western blot. Samples: Whole cell lysate from HEK293T (5, 15, and 50 µg) and HeLa (H; 50 µg) cells. Antibody: Affinity purified rabbit anti-JAM-A antibody orb1525814 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human JAM-A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-JAM-A antibody orb1525814 used for IP at 3 µg/mg lysate.

Detection of human JAM-A by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAM-A (Cat. No. orb1525814) used at a dilution of 1: 200 (1µg/ml). Detection: DAB