JAB1/COPS5 Antibody

• Catalog Number: orb308785
• Category: Antibodies
• Description: JAB1/COPS5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human JAB1 recombinant protein (Position: M8-S334). Human JAB1 shares 99.4% amino acid (aa) sequence identity with mouse JAB1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 37579 MW
• UniProt ID: Q92905
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: COP9 signalosome complex subunit 5;SGN5;Signalosom
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of JAB1 using anti-JAB1 antibody.JAB1 was detected in immunocytochemical section of A431 cell.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

Flow Cytometry analysis of U20S cells using anti-JAB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U937 cells using anti-JAB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-JAB1 antibody.Lane 1:Raji cell; 2:K562 cell; 3:PC-3 cell; 4:HeLa cell; 5:human A549 cell; 6:human HEK293 cell; 7:rat brain tissue; 8:rat heart tissue; 9:mouse brain tissue; 10:mouse heart tissue; 11:mouse SP2/0 cell.

IHC analysis of JAB1 using anti-JAB1 antibody. JAB1 was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of JAB1 using anti-JAB1 antibody. JAB1 was detected in a paraffin-embedded section of mouse testis tissue.

IHC analysis of JAB1 using anti-JAB1 antibody. JAB1 was detected in a paraffin-embedded section of rat testis tissue.