IWS1 Antibody

• Catalog Number: orb1522677
• Category: Antibodies
• Description: IWS1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: IWS1
• UniProt ID: Q96ST2
• NCBI: NP_060439.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: interacts with Spt6; IWS1 homolog; IWS1-like prote
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human IWS1 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, Hep-G2, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IWS1 antibody (orb1522677) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse IWS1 by western blot. Samples: Whole cell lysate (10 µg) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IWS1 antibody (orb1522677) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human IWS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IWS1 antibody (orb1522677) used for IP at 6 µg per reaction. IWS1 was also immunoprecipitated by a previous lot of this antibody (orb1522677) and a second antibody against a different epitope of IWS1.