ITPA Antibody

• Catalog Number: orb1264386
• Category: Antibodies
• Description: ITPA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Mouse, Rat, Xenopus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ITPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ITPA.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: ITPA
• UniProt ID: Q9BY32
• NCBI: Q9BY32
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Inosine triphosphate pyrophosphatase {ECO:0000255|
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Fluor 554 (red) conjugated Phalloidin (red).

ITPA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate (from left to right), using ITPA Antibody at 1:1000 at each lane.