ITLN1 Antibody / Intelectin 1 / Omentin

• Catalog Number: orb699752
• Category: Antibodies
• Description: Human intelectin-1 (ITLN-1) is a novel identified galactose binding lectin that is usually expressed in the heart, small intestine, and colon. In normal tissues, human intelectin-1 was mainly secreted from gastrointestinal goblet cells along with mucus into the intestinal lumen, and it was also expressed, to a lesser extent, in mesothelial cells and urinary epithelial cells. It is overexpressed in human malignant pleural mesothelioma (MPM). In contrast, sarcomatoid-type MPMs, biphasic MPMs, and poorly differentiated MPMs are rarely positive for intelectin-1, and it is not expressed in other cancers, except in mucus-producing adenocarcinoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ITLN1/4065
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 16-141 from the human protein was used as the immunogen for the Intelectin 1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Intelectin 1 antibody is available for research use only.
• UniProt ID: Q8WWA0
• Storage: Store the Intelectin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Intelectin 1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon with Intelectin 1 antibody (clone ITLN1/4065). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Intelectin 1 antibody. These results demonstrate the foremost specificity of the ITLN1/4065 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Intelectin 1 antibody (clone ITLN1/4065) as confirmation of integrity and purity.