ITGB4/Integrin Beta 4 Antibody

SKU: orb1538156

This product can be custom made

Description

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	FC, IHC, IHC-Fr, IHC-P, WB
Dilution Range	Flo, IHC, IHC-Fr, IHC-P (20 µg/ml), WB
Reactivity	Human
Application Notes	Further information: This antibody is for use in Western Blot only under non-reducing conditions. Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 20 ug/ml. Tested by flow cytometric analysis of A549 cell line. Antibody can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	439-9B
Immunogen	Human ITGB4
Target	ITGB4 / Integrin Beta 4
Purification	Affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.. Sourced from tissue culture supernatant
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ITGB4, CD104, Integrin beta-4, Integrin Beta 4, gp150, CD104 antigen, Integrin beta-4 subunit, Integrin, beta 4

Quality Guarantee

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