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Catalog Number | orb1538156 |
---|---|
Category | Antibodies |
Description | ITGB4 / Integrin Beta 4 Antibody (clone 439-9B) |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | 439-9B |
Tested applications | FC, IHC, IHC-Fr, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2b |
Immunogen | Human ITGB4 |
Concentration | 0.5 mg/ml |
Dilution range | Flo, IHC, IHC-Fr, IHC-P (20 µg/ml), WB |
Conjugation | Unconjugated |
Target | ITGB4 / Integrin Beta 4 |
Storage | Store at 4°C, avoid freeze-thaw cycles. |
Buffer/Preservatives | Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.. Sourced from tissue culture supernatant. Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.. Sourced from tissue culture supernatant |
Alternative names | ITGB4, CD104, Integrin beta-4, Integrin Beta 4, gp Read more... |
Note | For research use only |
Application notes | Further information: This antibody is for use in Western Blot only under non-reducing conditions. Immunohistochemistry: LS-B3778 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3778 was determined to be 20 ug/ml. Tested by flow cytometric analysis of A549 cell line. Antibody can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. |
Expiration Date | 12 months from date of receipt. |
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