ITGB3 (Phospho-Y785) antibody

• Catalog Number: orb393088
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ITGB3 (Phospho-Y785)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y785 of human CD61 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ITGB3
• Entrez: 16416, 3690
• UniProt ID: O54890, P05106
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: GP3A; Integrin beta-3; Platelet membrane glycoprot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD61 (Phospho-Y785) expression in U87MG (A), PC3 (B) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 110 kD)

Immunofluorescent analysis of CD61 (Phospho-Y785) staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Direct ELISA antibody dose-response curve using Anti-CD61 (Phospho-Y785) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.