You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1259324 |
---|---|
Category | Antibodies |
Description | ITGAM Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD11B (NP_001139280.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 180kDa |
Target | ITGAM |
UniProt ID | P11215 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Integrin alpha-M, CD11 antigen-like family member Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CD11B antibody (orb1259324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of rat bone marrow using CD11B antibody (orb1259324) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of RAW264.7 cells using CD11B antibody (orb1259324) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 cells using CD11B antibody (orb1259324) at dilution of 1:100. Blue: DAPI for nuclear staining.
FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating