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Catalog Number | orb1671991 |
---|---|
Category | Antibodies |
Description | ITGA5 Biosimilar Antibody |
Clonality | Recombinant |
Clone Number | M200 (Volociximab) |
Tested applications | Blocking, ELISA, FC, IF, IHC |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | Heterodimeric α5β1Fc fusion protein |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | ITGA5 |
UniProt ID | P08648 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Fibronectin receptor, CD49 antigen-like family mem Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volocixim449). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of M200 (orb1671991) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671991- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volocixim449) PBMC's (A) and U93 cells (B) were stained with unimmunized r antibody (black line) or the rmeric version of M200 (Volocixim91 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
Recombinant | |
Unconjugated |
Blocking, ELISA, FC, IF, IHC | |
Human | |
Recombinant | |
Unconjugated |
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