ITGA4 Antibody

• Catalog Number: orb1239641
• Category: Antibodies
• Description: ITGA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Integrin alpha 4 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Integrin alpha 4.The immunogen is located within amino acids 220 - 270 of Integrin alpha 4.
• Concentration: 1 mg/mL
• Dilution range: Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 μg/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ITGA4
• UniProt ID: P13612
• NCBI: NP_000876
• Storage: Integrin alpha 4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Integrin alpha 4 Antibody: IA4, CD49D, Integrin al
• Note: For research use only
• Application notes: Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 μg/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Integrin alpha 4 in rat spleen tissue lysate with Integrin alpha 4 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 µg/mL.