ITCH antibody

• Catalog Number: orb19579
• Category: Antibodies
• Description: Goat polyclonal antibody to ITCH
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 98.7, 102.7; 86.4
• Target: ITCH / AIF4
• Entrez: 83737
• Protein Sequence: EIKSHDLKPNGGN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ITCH antibody, anti AIF4 antibody, anti itchy
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3). Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19579 (0.03ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of A431 cells using ITCH antibody

Immunofluorescence analysis of U2OS cells using ITCH antibody

Flow cytometric analysis of HeLa cells using ITCH antibody