ITCH/AIP4 Mouse Monoclonal Antibody

SKU: orb3086035

Description

The ITCH/AIP4 Mouse Monoclonal Antibody (Carrier-free) is a Carrier-free antibody raised in mouse. It is immunogen affinity purified and suitable for use in FC, IHC, and WB applications. This antibody targets the E3 ubiquitin-protein ligase Itchy homolog in Human, Mouse, and Rat samples.

Research Area

Cancer Biology, Infectious Disease & Virology, Metabolism Research, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x^6 cells, Human
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: B7G34
• Immunogen: E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).
• Target: E3 ubiquitin-protein ligase Itchy homolog
• Molecular Weight: 103 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AIF4; AIP4; ITCH; NAPP1; NFE2 associated polypeptide 1

Quick Database Links

Gene Symbol

E3 ubiquitin-protein ligase Itchy homolog

UniProt

Q96J02