ISL2 Antibody (N-term)

• Catalog Number: orb1934810
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ISL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ISL2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q96A47
• MW: 39768 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000
• Antibody Type: Primary Antibody
• Clone Number: RB36036
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Insulin gene enhancer protein ISL-2, Islet-2, ISL2
• Note: For research use only
• NCBI: NP_665804.1

ISL2 Antibody (N-term) western blot analysis in A2058 cell line lysates (35 ug/lane). This demonstrates the ISL2 antibody detected the ISL2 protein (arrow).

Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term). HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). ISL2 immunoreactivity is localized to Nucleus significantly.