IRS1 (phospho-S312) antibody

• Catalog Number: orb318896
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IRS1 (Phospho-S312)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S312 of human IRS1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IF/ICC: 1:100-1:500, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: IRS1
• Entrez: 25467, 16367, 3667
• UniProt ID: P35569, P35568, P35570
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Insulin receptor substrate 1 antibody, anti I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IRS1 (Phospho-S312) expression in HepG2 (A), Hela (B), mouse spleen (C) whole cell lysates. (Predicted band size: 131 kD; Observed band size: 180 kD)

Immunofluorescent analysis of IRS1 (Phospho-S312) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.